
pDC315不带荧光的过表达腺病毒
产品名称: pDC315不带荧光的过表达腺病毒
英文名称: pDC315不带荧光的过表达腺病毒
产品编号: BiovectrePDC315
产品价格: 0
产品产地: Biovector Inc. USA
品牌商标: Biovector, Addgene, ATCC, Invi
更新时间: null
使用范围: null
Biovector Co.,LTD
- 联系人 :
- 地址 : 北京市西直门外大街19号BioVector NTCC典型培养物保藏中心
- 邮编 : 100080
- 所在区域 : 北京
- 电话 : 189****8599 点击查看
- 传真 : 点击查看
- 邮箱 : Biovector@163.com
Order ID |
Name |
Description |
BiovectrePDC315 |
pDC315 |
DNA.15uL,AmpR.Storage:-20℃ |
不带荧光的过表达腺病毒 客户如果做动物实验,有时候会要求不带荧光
Transformation of plasmid DNA to competent E. Coli cells
- Thaw competent cells on ice. 20–200µL per tube
- Add max. 1-3µL of plasmid
- Mix very gently!
- Incubate the tubes on ice for 30 min
- Heat shock the cells for 45 sec to 2 min at 42°C
- Place the tubes immediately on ice for at least 2 min
- Add 800µL of SOC medium to each tube
- Incubate for 1 hour at 37°C and shake vigorously
- Spin down briefly and remove most supernatants
- Resuspend cell pellet with the rest SOC medium in the tube by pipetting
- Plate out the suspension on a LB agar plate containing the appropriate antibiotic. Incubate the plates overnight at 37°C